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HIV-infected injection drug users (120) were randomly allocated to receive vitamin A or placebo. We find that homozygous mutant ES cells differentiate normally in vitro, giving rise to cardiomyocytes, myotubes, and smooth muscle cells in addition to many other cell types. Increased Factor V Leiden frequency is associated with venous thrombotic events among young Brazilian patients. An increased or steep learning curve has been described in the initial application of this technique.

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Hamiltonian description of nonlinear propagation in optical fibers. Acid glycosidase and arylsulfatase activities of human cerebrospinal fluid as measured by concanavalin A-sepharose affinity chromatography. Prevalence of drug resistance genotypes causing broad cross-resistance to nucleos(t)ide analogues.

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Models of potassium channel inactivation: implications for open channel block. This therapeutic approach implies a high frequency of hypoglycaemic events which can be disturbing when hypoglycaemia unawareness is involved.

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The data show that long duration mild hyperthermia (LDMH) at 40, 41 and 42 degrees C when given before acute irradiation resulted in increased radiosensitization with heating time. Spontaneous regression to an infant age level during self-hypnosis. The pregnancy rate following spontaneous intercourse or insemination was higher in those women in whom ipsilateral transport could be demonstrated. Role of arthrography in imaging of the temporomandibular joint in 1992 Spectroscopic studies of the oxidation-reduction properties of three forms of ferredoxin from Desulphovibrio gigas.

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Monitoring death and cancer in children born to HIV-infected women in England and Wales: use of HIV surveillance and national routine data. Bilateral retinal detachments developed in a renal allograft patient several months after the onset of cytomegalovirus retinitis. The data obtained from observational and epidemiologic studies is scanty, conflicting, and limited. Hepatic trauma: CT findings and considerations based on our experience in emergency diagnostic imaging.

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Arsenic trioxide treatment, in contrast, significantly increased the percentage of apoptotic T-lymphocytes from asthmatic patients, but slightly affected the cells from the control group. The majority of segments were devoid of any adhering tissue other than erythrocytes and polymorphonuclear cells. Detection of anti-human cell antibodies in sera from macaques immunized with whole inactivated virus. A 50-year-old man was admitted with acute inferior and anterior myocardial infarction. The molecular mechanism of androgen-independent growth of prostate cancer after androgen ablation was explored in LNCaP cells.

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The human genome has become a roadmap for elucidation of the pathophysiology of disease. One depends on the presence of allergen-specific IgE/IgG1 and allergen, and a second is mediated by allergen-independent inflammation of the lung. These findings suggest that TGF-beta1 produced by gastric cancer cells changes the morphology of mesothelial cells and may thus be closely associated with peritoneal dissemination. Evidence and consensus based Austrian guidelines for management of acute and chronic nonspecific backache

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Cellular interactions in the pathogenesis of lupus nephritis: the role of T cells and macrophages in the amplification of the inflammatory process in the kidney. In addition, oxidized proteins and lipids could stimulate cytokine release from macrophages through the activation of membrane receptors. We have previously demonstrated recombinational rescue of primer binding site (PBS)-impaired Akv murine leukemia virus-based vectors involving initial priming on endogenous viral sequences and template switching during cDNA synthesis to obtain PBS complementarity in second-strand transfer of reverse transcription (Mikkelsen et al., J. The tapered shape inlet has proven to be lower in the index of hemolysis (IH) than the straight shape.

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Analysis of promoter activity for the gene encoding pyruvate orthophosphate dikinase in stably transformed C4 flaveria species Cardiac catheterization showed no concomitant cardiac malformation. The large T antigen of simian virus 40 binds and inactivates p53 but not p73. Characterization of the lactococcal group II intron target site in its native host.

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Four monoclonal antibodies directed against ovine/bovine leucocyte antigens (Co.44B8, Co.11F10, Co.33B3 and Co.26F4) were produced and all recognized an antigen with an apparent molecular weight of 105 kDa under reducing conditions. Painstaking morphologic examination of a blood smear may at times lead directly to an important and unexpected diagnosis. Repeated freeze-thawing of cells in a lysis buffer was used to disrupt the cells and release genomic DNA.

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Although the differences between these two diagnostic groups were significant, the magnitude of the differences remained relatively modest. The search for neutralizing antibodies for foot-and-mouth disease has been undertaken by different methods.

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Population-based laboratory surveillance for tribe Proteeae isolates in a large Canadian health region. The aim of our study was to compare the in vitro angiogenic capacity, i.e. To further elucidate the regulation of SM-20 in smooth muscle, we cloned and analyzed the rat SM-20 promoter. This mini review will focus on these discoveries and emphasise how such BC proteins may be involved, through their interplay with cell cycle/DNA damage response pathways, in the development of human disease such as cancer.

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Only three cases had positive anti-HCV antibody, and they were thought to indicate past HCV infection. Effect of antibacterial agents on ammonia production within the intestine.

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Two children developed bacterial meningitis within five days of measles-mumps-rubella (MMR) immunisation. Origin in the collecting duct is suggested by medullary location, dysplasia of the epithelium in collecting ducts outside the tumor, and reactivity with antibody to high molecular weight cytokeratin and Ulex europaeus agglutinin. To determine the relative risk of prognostic factors for local disease control following RADPLAT. The data has been quite varied and several mediators and mechanisms have been considered responsible for interacting with the CNS, possibly because each plays a role at some level and their importance depends on the experimental system being examined.

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The slides depicted contexts from three areas of everyday life which partly contained visual cues related to a presented odor. We here show that AY-9944 (a 7-dehydrocholesterol reductase inhibitor) and U18666A (a 24-dehydrocholesterol reductase inhibitor) prevent PrP(Sc) from accumulating in prion-infected mouse neuroblastoma cells (ScN2a), with an ED50 of about 0.5 microM and 10 nM, respectively. Our findings suggest that renal tumors with VHL disease can be enucleated using a MTC safely and successfully without damaging renal function. For surgery, the cows were sedated and the base of the teat anaesthetized.

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Instead of using an opiate narcotic, ketorolac 60 mg IM was given 1 hour before induction as the analgesic portion of anesthesia. After that, the virus was detected almost continually from the central nervous system and abdominal parenchymatous organs in nearly all the hens examined up to the end of the observation period, or 21 days after inoculation. Moreover, we show that the 9-1-1 complex possesses a stimulatory effect on long patch base excision repair (LP-BER) reconstituted in vitro. Also evidence is presently available, that the permeability of the blood-brain barrier may be under active regulatory control.

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No systematic biases could be found in the studies to explain these differences. In contrast, informants tended to underestimate abilities of demented subjects, but not to a significant degree. Although very stringent photodynamic treatment conditions generate annexin V-positive RBCs, the assay will not detect more subtle membrane damage that is readily detected by other well-established methods. Constructing competitors of different sizes to the target may not eliminate heteroduplex formation when they share common sequence with the target as with the intron and deletion type competitors.

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These small cells are presumably of donor origin based on DNA fingerprinting analysis and based on fluorescence in situ hybridization for the Y chromosome in a sex-mismatched case. Dendritic cells (DC) isolated from lymph nodes of mice treated 24 hr earlier with trinitrochlorobenzene (TNCB) were capable of in vitro stimulation of lymphocytes from TNCB-sensitized mice, but not lymphocytes from mice sensitized to the preservative mixture of 5-chloro-2-methylisothiazolinone plus 2-methylisothiazolinone (MCI/MI). The pathophysiological changes include decreased cardiac output, systemic hypotension, decreased blood flow and O2 delivery to tissues, intense pulmonary vasoconstriction and hypertension, bronchoconstriction, increased permeability, pulmonary oedema, ventilation-to-perfusion inequalities, hypoxaemia, and haemoconcentration.

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Levofloxacin implants with predefined microstructure fabricated by three-dimensional printing technique. Optimization of silica-based media for antibody purification by protein A affinity chromatography. Molecular analysis of the somatostatin receptor subtype 2 in human glioma cells.

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Contrary to A2 mRNA which displays amastigote-stage-specific expression, A2rel gene expression is constitutive throughout the L. Data from the 3,534 patients enrolled in the TIMI II trial were analyzed to determine whether a history of antecedent angina before myocardial infarction identifies patients at high risk for subsequent ischemia and whether these patients might benefit from an invasive strategy. Regardless of the pathophysiology, these alterations in respiratory status represent an early warning sign of NEC. The radiographic study of the paraffin blocks confirmed the nature of this material, which was X-ray opaque, and this was corroborated histochemically with the rhodizonate technique. In the same time, 48 pedicle screwings were performed manually in the same region and on the same vertebral levels.

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The alveolar edema and ventilation:perfusion mismatch initiate a catastrophic downward spiral of worsening alveolar hypoxia. Pilot study of relapsed osteosarcoma and brain tumor with ifosfamide, carboplatin and etoposide (ICE therapy) We believe that this is the first reported fatality caused by M.

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By the inoculation of VSV into the culture, marked augmentation of antibody response to sheep erythrocytes (SRBC) was observed in the culture of spleen cells taken more than 3 days after the immunization with SRBC, suggesting that the VSV-susceptible suppressor cells were included in these spleen cells and the activity was eliminated by the effect of VSV. Orthostatic blood pressure decline in patients with this disease was significantly marked in comparison with that in 50 patients with lacunar infarction, especially in the males.

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The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography. However, an increase in other types of seizures was noted, as was an increase in EEG epileptiform discharges.

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Percentage of Ki-67 and c-myc positive cells correlated with each other and with grade of tumour, mitotic index, cellularity and survival time of patients, but not with the extent of necrosis. Strong leadership, effective communication, organized training sessions, established time frames, planned change, and tailored software are the essential factors to consider for development of a successful educational program.

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Patient age, prostate volume, total amount of energy and grade of outlet obstruction were categorized using the Akaike information criterion (AIC). The following topics are discussed in more detail: isolation of specific antigens by continuous elution-isoelectric focusing methods, production of recombinant antigens, nature of immunodominant antigens, potential use of heat shock proteins (HSPs) as diagnostic antigens, roles of specific IgE and circulating antigens (CA). Combined liver transplantation together with partial/total nephrectomy in patients with renal cell cancer and nonalcoholic steatohepatitis.

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This phylogenetic difference is consistent with the existence in catarrhine primates of a sweetness receptor containing two differently located hydrophobic recognition sites, one for the hydrophobic binding site of alitame, the other for the hydrophobic binding site of aspartame. The use of the D-STAT dry bandage for the control of vascular access site bleeding: a multicenter experience in 376 patients. We could find no net benefit of IAT in patients undergoing elective, infrarenal aortic surgery.

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HTLV-I was also detected in epithelial cells derived from mouthwash of the TSP/HAM patient. Patch closure of the aortic end of the tunnel eliminated left ventricular volume overload with immediate marked reduction of cardiomegaly.

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Three leukocyte classes (Fc gamma RI, II, and III) and one separate endothelial Fc gamma R class (FcRB) are defined which are expressed on hematopoietic and endothelial cells. An in vivo closed spinal window technique in rats was designed for observing the spinal microcirculation, such as the change of vessel diameter, leukocyte adhesion, and red blood cell (RBC) velocity, which has been very rarely examined in vivo in the spinal cord. All nonmutagenic complexes tested thus far have shown classical irreversibility, more negative reduction potentials, and an inability to relax supercoiled plasmid DNA. Cytogenetic analysis of both cultures revealed a rearranged chromosome 14 and several other chromosome aberrations, three of which may be used as a reliable means of identifying NAB cultures. Recombinant affinity purified BV-PLA2 was refolded, purified to homogeneity, and cleaved with kallikrein, resulting in a final yield of 8-9 mg of active enzyme per 1 of culture.

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Transient non-obstructing intussusception is known to occur in adults. The use of central venous catheters impregnated with minocycline and rifampin is associated with a lower rate of infection than the use of catheters impregnated with chlorhexidine and silver sulfadiazine. Early and aggressive surgical removal of the primary lesion followed by adjuvant therapy is recommended for the treatment of the disease. This study compares three molecular methods with each other and with phage typing.

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The pharmacokinetic parameters of DA-8159, a new erectogenic, were compared after intravenous and oral administration of the drug at a dose of 30 mg/kg to control rats and rats with acute renal failure induced by uranyl nitrate (U-ARF). Widespread antibiotic use has also resulted in selection for, and dissemination (especially in hospitals) of, multiresistant bacteria such as multiple-resistant Staphylococcus aureus (MRSA), expanded-spectrum beta-lactamase (ESBL) producing gram-negative bacteria, penicillin-resistant pneumococci and vancomycin-resistant enterococci (VRE). Application of superlattice multipliers for high-resolution terahertz spectroscopy. This study examined secular trends in HDL in relation to changes in other cardiovascular risk factors for the years 1981 through 1993 in the Pawtucket Heart Health Program (PHHP) study communities.

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The trend could be detected only in cores with accumulation rates greater than 0.7 mm year(-1). Both PF and cultured PMC superfusate induced a potent, rapidly reversible decrease in the shortening of isolated rat cardiac myocytes.

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But if triage category is used to help define ED case-mix groups for comparative or benchmarking processes, agreement on exact triage level and major system involved is important. Determinations as to the need for long-term anticoagulation require that a clinical assessment be made regarding the relative benefit in preventing thrombotic episodes versus the risk of increased bleeding. The reverse growth response occurred with increased supply of fertilizer: plant biomass rose with a larger increase in shoot mass than hypocotyl mass. The inosine levels in the CSF of the infants with any of the illnesses were significantly higher when compared with the controls.

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Esophageal atriogram in mitral disease: electromanometric recording of pressure curves The personality, psychopathological, and demographic differences between 330 alcoholic men referred for behavioral problems and 104 alcoholic men referred for medical problems were examined. The role of the renin-angiotensin system in kidney development is now widely accepted. Based on current data on Na/H exchanger isoforms (NHE-1 to 5), NHE-3 is the likeliest candidate for the apical membrane isoform.

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To describe eight patients with reflex sympathetic dystrophy in terms of clinical and laboratory characteristics and treatment. Oxidation of hydroxylamine to nitrite as an assay for the combined presence of superoxide anions and hydroxyl radicals. To ascertain whether primary resection in patients with obstruction from left colorectal carcinoma has advantages over staged resection in terms of morbidity and mortality. In presence of EDTA carboxycathepsin did not inactivate bradikinine. Prevention of reoxygenation injury by sodium salicylate in isolated-perfused rat liver.

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The flow rate was constant for a 15-minute sampling period when two to eight filters were used as a flow restrictor. Quality of care in postoperative pain management: what is realistic in clinical practice? Using two-dimensional nuclear magnetic resonance analysis, we now describe a new function for the Eps15 homology (EH) domain of EHD1 and show that it is capable of directly binding phosphatidylinositol moieties. The variants were characterized with respect to their catalytic activities and their abilities to bind to DNA and the S-adenosylmethionine (AdoMet) cofactor. Several factors, such as urinary tract infection (UTI), the site of the stone, pre-existing renal abnormalities, the number and intensity of the shock waves may influence the extent of the damage.

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THE DISTRIBUTION OF ADRENERGIC NERVE FIBRES TO THE BLOOD VESSELS IN SKELETAL MUSCLE. Thus, the aim of this review is to discuss current murine models of combined DM and atherosclerosis and to explore how these experimental systems are being utilized to gain mechanistic insights into diabetes-induced neointimal lesion development, as well as their potential use in evaluating the efficacy of new therapies. Utilization of agoutis in a research program of the National Institutes of Health necessitated the development of suitable methods for feeding, housing and collecting blood. Tsunami mortality estimates and vulnerability mapping in Aceh, Indonesia. The purpose of this pilot study was to develop a videofluoroscopic technique to evaluate dynamic residual limb-socket relationships during gait.

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Five patients who underwent testing with a Western blot assay were found to have antibodies against 4 antigens with molecular masses of 18, 21, 23, and 31 kd. Elevated PC-1 content in cultured skin fibroblasts correlates with decreased in vivo and in vitro insulin action in nondiabetic subjects: evidence that PC-1 may be an intrinsic factor in impaired insulin receptor signaling. A proton signal from the CH2 groups borne by free intracellular very long chain fatty acids can also be observed.